A RESEARCH OF THE ROLE OF MEMBRANE STRUCTURES IN PROCESSES OF NONSPECIFIC ADAPTATION OF THROMBOCYTES TO THE INFLUENCE OF A LOW-INTENSIVE HELIUM-NEON LASER IN THE CONDITIONS OF A MODEL EXPERIMENT

Mel'nikova Natal'ya Alekseevna, Candidate of biological sciences, associate professor, sub-department of normal and
pathological physiology with a course of hygiene, Ogarev Mordovia State University (68 Bolshevistskaya street, Saransk, Russia), n_melnicowa@mail.ru
Sedova Dina Georgievna, Candidate of biological sciences, associate professor, sub-department of normal and pathological physiology with a course of hygiene, Ogarev Mordovia State University (68 Bolshevistskaya street, Saransk, Russia), sedova_dg@mail.ru
Ulanova Tat'yana Vyacheslavovna, Candidate of medical sciences, associate professor, sub-department of normal and pathological physiology with a course of hygiene, Ogarev Mordovia State University (68 Bolshevistskaya street, Saransk, Russia), tatyana_304@mail.ru
Agenosova Ol'ga Gennad'evna, Candidate of biological sciences, associate professor, sub-department of cytology, histology and embryology with courses of medical biology and molecular biology of cells, Ogarev Mordovia State University
(68 Bolshevistskaya street, Saransk, Russia), olg.agen2014@yandex.ru
Evstifeeva Inna Aleksandrovna, Candidate of biological sciences, associate professor, sub-department of cytology, histology and embryology with courses of medical biology and molecular biology of cells, Ogarev Mordovia State University
(68 Bolshevistskaya street, Saransk, Russia), inna-evst@mail.ru

612.014.3; 612.014.4

DOI

10.21685/2307-9150-2018-1-9

Background. The low-intensity laser radiation is widely used as a physiotherapeutic method of impact on blood and its components. In too time remains up to the end not opened the mechanism of impact of light of an optical spectrum on fabrics of the internal environment. It is supposed that this influence has nonspecific character and is carried out, including, at the level of membranous structures of cages. By us it was examined influence of radiation of the helium-neon laser on a condition
of membranes of thrombocytes, their viability and the functional activity.
Materials and methods. As object of a research served the thrombocytes allocated from blood of donors. For irradiation of trombotsitarny suspension used light of the low-intensive LG-78 helium-neon laser, 0,02 MW with a wavelength of
632,8 nanometers in doses 1,2, 6,0, 12,0 and 24,0 J/cm2. Investigated the functional condition of thrombocytes, their viability, transmembrane potential and also compliance of changes of microviscosity of membranes to changes of ratios of saturated
fatty and unsaturated fatty acids, a cholesterin and phospholipids in fraction lipids of membranes.
Results. It is еstablished that the сhosen exposure doses exert the photomodifying impact on membranes of thrombocytes. Doses in 1,2, 6,0 and 12,0 J/cm2 successively lead to increase in a ratio among the content of the common phospholipids
and a cholesterin and also among unsaturated and saturated fatty acids of phospholipids.
The specified changes in general have positive character and are followed by reduction in microviscosity of membranes of thrombocytes within physiological norm, with synchronous increase of level of transmembrane potential, the viability
of thrombocytes and their potential aggregation activity remain. The dose in 24,0 J/cm2 caused reduction of a weight of phospholipide fraction in a bullet of the common lipids of membranes of thrombocytes, decrease in amount of unsaturated
fatty acids in them, increase of a share of a cholesterin. Also indicators of viabilityof thrombocytes decreased, a part of plates morphologically was in a condition of an incipient state of activation.
Conclusions. In an experiment it is established that the helium-neon laser causes in membranes of thrombocytes the photoeffects of nonspecific character leading to redistribution of membrane lipids, change of the liquid properties of a membrane allowing
to maintain viability of platelets and their functional activity. Increase in level of transmembrane potential in thrombocytes can demonstrate increase of intensity of energy consumption. In general effects helium-neon stimulation have character
of the “weak” physiological influences which are not leading alive systems, whether it be membranes or cells to a stressful condition, an overvoltage of their function systems or development of a parabiosis.

Key words

helium-neon laser, fatty acids, microviscosity of membranes, thrombocyte, transmembrane potential, cholesterol, phospholipids, vitality

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